Are you making Antisense?

oligonucleotide synthesis: Oligonucleotides are chemically synthesized using nucleotides, called phosphoramidites, normal nucleotides that have protection groups: preventing amine, hydroxyl groups and phosphate groups interacting incorrectly. One phosphoramidite is added at the time, the product’s 5′ phosphate is deprotected, and a new base is added, and so on (backwards); at the end, all the protection groups [...]